Flygut-seq is a transcriptomic resource that complements the original Flygut website. In Flygut, you can find an interactive database that hosts an atlas of Drosophila gut regions, and provides microarray data of the main regions. In Flygut-seq, we now provide an additional genomic resource, that includes cell specific, regions specific RNAseq of the main midgut regions. In addition we provide cell-specific gene regulation in response to oral infection with the entomopathogenic bacteria Pseudomonas entomophila.

This resource is associated with the publication: Regional cell-specific transcriptome mapping reveals the regulatory complexity of the adult Drosophila midgut. Devanjali Dutta, Adam J. Dobson, Philip L.Houtz, Christine Gläßer, Jonathan Revah, Jerome Korzelius, Bruce A. Edgar and Nicolas Buchon.
FlySick-seq is a transcriptomic resource for the study of the host response to infection in D. melanogaster. In Flysick, you can find gene expression data for every gene following sterile wound, inoculation with heat-killed bacteria, and infection with 10 different bacteria: M. luteus, E. coli, S. marcescens Type, Ecc15, P. rettgeri, E. faecalis, S. aureus. P. sneebia, S. marcescens Db11, and P. entomophila. We provide expression data for the following time points: 12, 36, and 132 hours post-infection.

This resource is associated with the publication: Comparative transcriptomics reveals CrebA as a novel regulator of infection tolerance in D. melanogaster. Katia Troha, Joo Hyun Im, Jonathan Revah, Brian P. Lazzaro and Nicolas Buchon.

FlySexsick-seq: Male and female gene expression upon infection
FlySexsick-​seq is a transcriptomic resource for the study of sexually dimorphic responses upon infection. In FlySexsick, you can find gene expression data for every gene in unchallenged condition (UC) and following injection of the Gram-negative bacterium Providencia rettgeri. We provide expression data at 8 hours post-infection.

This resource is associated with the publication: The Toll pathway underlies sexual dimorphism in resistance to both Gram-negative and positive-bacteria in Drosophila David F. Duneau, Hannah C. Kondolf, Joo Hyun Im, Gerardo A. Ortiz , Christopher Chow, Michael A. Fox, Ana T. Eugénio, J. Revah, Nicolas Buchon, Brian P. Lazzaro.

​Aegypti-Atlas

Aegypti-Atlas is an interactive database that provides a comprehensive atlas of the transcriptome of different regions/tissues (head, thorax, abdomen, Malpighian tubules, ovaries, gut) of the female yellow fever mosquito, Aedes aegypti. In addition, you will find region-specific RNAseq data for the mosquito gut (crop, proventriculus, anterior midgut, posterior midgut, and hindgut).

This resource is associated with the publication: A transcriptomic atlas of Aedes aegypti reveals detailed functional organization of major body parts and gut regional specializations in sugar-fed and blood-fed adult females. Bretta Hixson, Xiao-Li Bing, Xiaowei Yang, Alessandro Bonfini, Peter Nagy, Nicolas Buchon​.